Splenic immune response to Plasmodium infection initiated by two different routes

Plasmodium chabaudi parasite obtained from recently mosquito transmitted infections give rise to an attenuated blood-stage infection when compared to serially blood passaged parasites. We compared the immune response in the spleens induced by these two types of parasites in order to determine what may cause this attenuation. Spleens were taken at several timepoints during the blood-stage infection and RNA-seq analysis was performed. 5 Time points - (Days 1, 2, 3, 4, 6) and 2 infection conditions (RMT and SBP). Each group contains 4 replicates, using age/sex matched C57BL/6 mice. Controls/Naïve mouse with no infection were age/sex matched.