Altered Circular RNA Expression in Patients with Repeated Implantation Failure

To evaluate differentially expressed circRNAs in patients with recurrent implantation failure, we used circRNA microarrays to screen circRNA expression profiles in endometrial biopsies from 6 women with recurrent implantation failure and controls. Our study revealed for the first time a unique set of circRNA expression signatures in the endometrial tissue of patients with repeated implantation failure, which may provide new molecular candidates for the diagnosis and clinical treatment of embryo implantation failure. We performed circRNA microarray analysis on endometrial tissue samples from six implantation windows and applied bioinformatics analysis to investigate these differentially expressed circRNAs. In addition, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to confirm these results.