Stem cell gene expression profiling of primary mammary tumors derived from orthotopic xenograft tumors treated with Sulforaphane (SFN) or control I

In this study, we explored stem cell markers profile of mammary tumor initiation and growth derived from a human breast cancer cell line (MDA-MB-231 D3H1 Luci). In this dataset, we include expression data from primary mammary tumors pre-treated with Sulforaphane for 2 weeks (daily i.p. injection of 50mg SFN/kg) and kept treatment after cell injection for more 3 weeks and Saline 0.9% as a Control. In total, 36 samples were analyzed. We built a customized NanoString nCounter® Gene Expression Codeset of 107 genes and controls that contained significant embryonic core stem cell genes and epithelial-mesenchymal transition (EMT)-Mesenchymal Epithelial Transition (MET) markers. We generated the following pairwise comparison using nSolver version 2.070: SFN vs. Saline. Genes with a p-value < 0.05 and an absolute fold-change of 1.5 were selected.