Data from: Topological, chemical, and electro-optical characteristics of riboflavin-doped artificial and natural DNA thin films

DNA is considered as a useful building bio-material, and it serves as an
efficient template to align functionalized nanomaterials. Riboflavin
(RF)-doped synthetic double-crossover DNA (DX-DNA) lattices and natural
salmon DNA (SDNA) thin films are constructed using substrate assisted
growth and drop-casting methods, respectively, and their topological,
chemical, and electro-optical characteristics were evaluated. The critical
doping concentration of RF ([RF]C, ~5 mM) at given concentrations of
DX-DNA and SDNA were obtained by observing the phase transition (from
crystalline to amorphous structures) of DX-DNA and precipitation of SDNA
in solution above [RF]C. [RF]C are verified by analyzing the atomic force
microscopy images for DX-DNA and current, absorbance, and
photoluminescence for SDNA. We study the physical characteristics of
RF-embedded SDNA thin films, which are the Fourier transform infrared
(FTIR) spectrum to understand the interaction between the RF and DNA
molecules, current to evaluate the conductance, absorption to understand
the RF binding to the DNA, and photoluminescence (PL) to analyze the
energy transfer between the RF and DNA. The current and UV absorbance band
of SDNA thin films decrease up to [RF]C followed by an increase above
[RF]C. In contrast, the PL intensity illustrates the reverse trend, as
compared to the current and UV absorbance behavior as a function of the
varying [RF]. Due to the intense PL characteristic of RF, the DNA lattices
and thin films with RF might offer immense potential to develop efficient
bio-sensors and useful bio-photonic devices.