Fig2D_RACE_STOML2_YKT6_ExtFig3E_TUBA1B_R3_LG289.sgd
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HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X or vector and luciferase reporters with or without 99 bp inserted sequences from the STOML2, YKT6 and TUBA1B genes. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.
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Gaglia, Marta创建时间:
2023-01-19



