Transcriptomic changes induced by Curcumin loaded solid nanoparticles and radiation in breast cells

In breast cancer (BC) care, radiotherapy is considered an efficient treatment. However, approximately 90% of BC-related deaths are due to the metastatic tumor progression. Then, it is strongly desirable to improve tumors radiosensitivity using molecules with synergistic action. In this study, we developed curcumin-loaded solid nanoparticles (Cur-SLN) in order to increase curcumin bioavailability and evaluated their radiosensitizing ability in comparison to curcumin free (Cur), by using an in vitro approach on BC models. Precisely, here we analyzed the transcriptomic profiles induced by Cur-SLN treatments, in non tumorigenic MCF10A and tumorigenic MCF7 and MDA-MB-231 BC cell lines, highlighting the networks responsible of its radiosensitization ability. Curcumin loaded- SLN can be suggested in future preclinical and clinical studies to test its concomitant use during radiotherapy treatments with the double implications of being a radiosensitizing molecule against cancer cells, with a protective role against IR side effects. We performed two-color hybridization experiments using Agilent Technologies whole human genome 4x44K microarrays after curcumin and radiation (photons) treatment in non- tumorigenic (MCF10A) and in tumorigenic breast (MCF7 and MDA-MB-231) immortalized cell lines. New Samples were named as follow: MCF10A_curcumin_2Gy; MCF7_curcumin_2Gy; MDA-MB-231_curcumin_2Gy