Amblyomma testudinarium Raw sequence reads

In this study, we reported two featured series of rRNA-derived RNA fragments (rRFs) from the small RNA sequencing (sRNA-seq) data of Amblyomma testudinarium using the Illunima platform. Two series of rRFs (rRF-5 and rRF-3) were precisely aligned to the 5'' and 3'' ends of 5.8S and 28S rRNA genes. The rRF-5 and rRF-3 series were significantly highly expressed than the rRFs located in the body of the rRNA genes. In addition, these series contained perfectly matched reads, the lengths of which varied progressively with 1-bp differences. These results indicated the rRF-5 and rRF-3 series were not random intermediates or products during rRNA degradation, but could constitute a new class of small RNAs that deserve further investigation.