Confounding factors in the analysis of the transcriptome response to UV exposure in skin of wild-type and human p53-variant mice. Mus musculus

An in-vivo experiment with UV-B exposure of the skin of nude, male mice, with and without human-derived p53 variants in their genome to investigate the cellular responses upon UV-induced DNA damage. Essentially, 4 replicate mice were used for each level of UV-exposure. For each mouse a biopsy of the skin was sampled 5-6 times at different recovery time points after UV-pulse exposure, which resulted in paired samples. In total 132 treated and 132 untreated samples were taken from 64 male mice. Overall design: 264 skin biopsies were taken from 48 mice on 10 timepionts and for 3 different UV-B doses. 3 different genotypes of mice were used, Wild-type (WT; p53+/+), p5372R/72R, and p5372P/72P. Later, it was determined that eight samples came from female mice instead of male mice. Therefore, the eight samples were excluded further analyses (no processed data were generated from these arrays).