RNA-seq of in vitro cultured Sox9+ mouse lung progenitors cells from E12.5 embryos compared to freshly isolated cells and control adult lung epithelial cells

The study aims to show that Sox9+ Chd1+ mouse embryonic lung progenitors can be isolated and expanded long-term in 3D culture while maintaining their multipotency. in vitro cultured Sox9+ Chd1+ lung progenitors transcriptionally resemble their in vivo counterparts and show significant difference from adult lung epithelial (Cdh1+ and EpCAM+) and non-epithelial (Cdh1- and EpCAM-) cells本研究旨在证实，可分离Sox9+ Chd1+小鼠胚胎肺祖细胞，并在三维培养体系中长期扩增，同时维持其多向分化潜能。经体外培养的Sox9+ Chd1+肺祖细胞，其转录组特征与体内同源细胞高度相似，且与成年肺上皮细胞（Cdh1+及EpCAM+）、非上皮细胞（Cdh1-及EpCAM-）存在显著差异。