File S1 - Monolayer Stress Microscopy: Limitations, Artifacts, and Accuracy of Recovered Intercellular Stresses

Supporting Information S1–S8. Details of experimental and mathematical approach and accuracy assessment for two other monolayer geometries of interest. The supporting information contains several topics which includes, protocol for mapping stresses (S1), Fourier representation of three dimensional Boussinesq solution (S2), data for alignment between maximum principal orientation and cell orientation (S3), accuracy assessment for two other monolayer systems of interest (S4), effect of adjacent free edges on rate of decay of boundary artifacts (S5), procedure for mapping decay of boundary artifacts (S6), effect of substrate thickness on substrate tractions (S7), and enlarged images of selected results (S8). (PDF)