Single nuclei RNA-seq analysis of control and Dnmt3a conditional knockout (cKO) of retinal ganglion cells at 2 days post optic nerve crush.

Dnmt3a deficiency in retinal ganglion cells promotes axon regeneration in an optic nerve crush mouse model. To investigate the role of Dnmt3a in axon regeneration, retinal ganglion cell nuclei were sorted from control and retinal ganglion cell-specific Dnmt3a knockout mice at 2 days post optic nerve crush and applied for single nuclei RNA-seq analysis by 10X Genomics technology. snRNA-seq were performed for retinal ganglion cell nuclei isolated from the retinas of 6 cKO and 6 sex-matched control mice with optic nerve crush, using FACS. Total 8,000~9,000 cells were sequenced to an average of 22,000-30,000 reads/cell per dataset.