Doubling times of <i>E. coli</i> MG1655 expressing FbFPs, GFP, and YFP.
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PpFbFP, EcFbFP, iLOV, and YFP were expressed in E. coli MG1655 using an IPTG-inducible phage T5 promoter harbored in a medium copy plasmid (pQE80L). In addition, iLOV and GFP were expressed using a constitutive phage lamda promoter in a low copy plasmid (pAM06-tet). Cells were grown in M9 media using glycerol or glucose as the carbon source and doubling times were estimated over the logarithmic phase of cell growth corresponding to 0.4< A600nm <0.8.
创建时间：
2013-05-31
