Comparison of relative whole cell fluorescence of s-GFP, n-GFP and deletion variants.

The relative fluorescence (in arbitrary units) is defined as the whole cell fluorescence compared with fluorescence of cells expressing s-GFP[a] and n-GFP[b] respectively. All the fluorescence values were normalized by the O.D600 nm of expressed cells. [c]Deletion of preceding residues in primary structure of n-GFP resulted in loss of fluorescence and experiments were not carried out. [+]Reported elsewhere [6] that these loop deletions makes the protein non-functional.