five

Enriched VD2s stimulated with opsonized iRBC

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http://flowrepository.org/id/FR-FCM-Z325
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PBMC were enriched for VD2 T cells and stimulated with iRBC + hyper-immune or naive sera to generate opsonized and unopsonized antigen. IFNy and CD107a was measured as a result of stimulation to determine the addition of hyper-immune sera increased the number of VD2s responding to malaria antigen. VD2s were enriched to eliminate an effect of other CD-16 expressing cell types on VD2 stimulation.
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2020-10-01
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