scRNAseq gene expression profile of zebrafish jaw joint cells after IOM ligament transection.

Adult zebrafish have the capacity to regenerate craniofacial ligament tissue following a complete transection injury. How this robust skeletal regeneration is achieved remains undefined. Here, we use single cell RNA sequencing to profile RNA expression from FACS sorted cranial neural crest lineage and non-cranial neural crest lineage cells (including skin and immune populations) in the first 3 days after ligament injury. To understand the cellular contributors and molecular regulation of craniofacial ligament regeneration in adult zebrafish, we collected jaw joint cells for single cell RNA sequencing following ligament injury. After IOM ligament transection injury and in uninjured and SHAM surgical controls, neural crest lineage cells (DsRed) and non-neural crest lineage cells (BFP) were isolated using Fluorescence-activated cell sorting (FACS) from jaw joints microdissected from Sox10:Cre;actb2:loxP-BFP-STOP-loxP-DsRed transgenic fish and analyzed using scRNAseq.