Transcriptomic analysis in the intestine of IL-17A/F1-knockout medaka, Oryzias latipes.

In mammals, interleukin (IL)-17A and IL-17F are hallmark inflammatory cytokines, which are expressed by Th17 cells, and play important roles in protection against infection as well as in intestinal mucosal immunity. Although fish IL-17A and IL-17F homologs named as IL-17A/F have been identified, and their functional aspects are still poorly understood, especially in intestinal mucosal immunity. In this study, IL17A/F1-mutated (IL17AF1-KO-) medaka (Oryzias latipes) was established using CRISPR/Cas system, and a 7-bp deletion was confirmed in the IL-17A/F1 gene. To understand the roles of IL-17A/F in the mucosal tissues of fish, the transcriptomic analysis (RNA-Seq) on the intestine of IL17AF1-KO-medaka was performed using a MiSeq next generation sequencer.