Arabidopsis thaliana Transcriptome or Gene expression

Ten-day-old seedlings were dehydrated on dry filter paper in Petri dishes till loss of 40% fresh weight and then incubated for 2 h in sealed plastic bags to prevent further water loss. Total RNA was extracted using the RNeasy mini kit (Invitrogen), and DNA was cleaned by DNase I (New England Biolabs). About 2 to 4 µg of cleaned total RNA was used to construct RNA-seq libraries by using an Illumina Whole Transcriptome Analysis Kit following the standard protocol (Illumina, HiSeq system) and sequenced on the HiSeq 2000 platform.