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Rapid and easy method for in vitro determination of transcription factor binding core motifs

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Figshare2019-08-30 更新2026-04-29 收录
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We introduce a rapid method for easily elucidating transcription factor (TF) cis-elements by adopting a highly efficient in vitro protein synthesis method and identifying protein-DNA interactions using PCR. We determined two cis-elements for plant TFs using this method, and the results confirmed our method as an easy and time-saving alternative for elucidating TF cis-elements using common laboratory procedures.

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2019-08-30
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