Transcriptomic Characterization of Human Beige Adipocyte Differentiated From Adipose Mesenchymal Stem Cells

Beige adipocytes are a distinct type of thermogenic fat cells in human. Since beige adipocytes are distributed sporadically within white adipose, characterization of human beige adipocytes has long been a problem. In this study, we reported a rapid and roboust method in generating human beige adipocyte with chemically defined medium and RNA-Seq was perfomed to reveal the molecular characterization of derived human beige adipocytes. mRNA profiling of adipose derived mesenchymal stem cells and in vitro differentiated beige adipocytes were generated by RNA-Seq, in duplicate, using illumina NextSeq platform. mRNA profile of in vitro differentiated white adipocytes were also generated by same method and serve as control.