Raw Data of LC-MS on Culex pipiens pallens after selection to three insecticide

Abstract Background: Mosquito control based on chemical insecticides is
considered as an important element of the current global strategies for
the control of mosquito-borne diseases. Unfortunately, the development of
insecticide resistance of important vector mosquito species jeopardizes
the effectiveness of insecticide-based mosquito control. In contrast to
target site resistance, other mechanisms are far from being fully
understood. Methods: Global protein profiles among cypermethrin-resistant,
propoxur-resistant, dimethyl-dichloro-vinyl-phosphate (DDV)-resistant and
susceptible strain of Culex pipiens pallens were obtained and proteomic
differences were evaluated by using isobaric tags for relative and
absolute quantification (iTRAQ) labeling coupled with liquid
chromatography/tandem mass spectrometric (LC-MS/MS) analysis. Results: A
susceptible strain of Cx. pipiens pallens showed elevated resistance
levels to after 25 generations of insecticide selection, through iTRAQ
data analysis detected 2,502 proteins, of which 1,513 were differentially
expressed in insecticide-selected strains compared to the susceptible
strain. Finally, midgut differential protein expression profiles were
analyzed, and 62 proteins were selected for verification of differential
expression using iTRAQ and parallel reaction monitoring strategy,
respectively. Conclusions iTRAQ profiles of adaptation selection to three
insecticide strains combined with midgut profiles revealed that multiple
insecticide resistance mechanisms operate simultaneously in resistant
insects of Cx. pipiens pallens. Significant molecular resources were
developed for Cx. pipiens pallens, potential candidates were involved in
metabolic resistance and reducing penetration or sequestering insecticide.
Future research that is targeted towards RNA interference of the
identified metabolic targets, such as cuticular proteins, cytochrome
P450s, glutathione S-transferases and ribosomal proteins proteins and
biological pathways (drug metabolism - cytochrome P450, metabolism of
xenobiotics by cytochrome P450, oxidative phosphorylation, ribosome) could
lay the foundation for a better understanding of the genetic basis of
insecticide resistance in Cx. pipiens pallens.