<i>Anemarrhenae rhizoma</i> combined with <i>Trichosanthis radix</i> ameliorated type 2 diabetes in rats via the PI3K-Akt signalling pathway based on network pharmacology analysis

By combining network pharmacological analysis and animal model experiments, the research aims to understand the pharmacological profile and mechanisms of <i>Anemarrhenae rhizoma</i> (Zhimu) and <i>Trichosanthis radix</i> (Tianhuafen) herb-pair (AT) in type 2 diabetes mellitus (T2DM) treatment. The results of GO and KEGG enrichment analyses suggested that AT exerted pharmacological effects on T2DM by regulating PI3K/Akt signalling pathway. Finally, molecular docking confirmed a strong binding affinity between AT with the core target proteins. Further <i>in vitro</i> experiments have confirmed that AT treatment significantly reduced body weight, blood glucose, and insulin levels, also blocking the activation of the PI3K/Akt signalling pathway.

本研究结合网络药理学分析与动物模型实验，旨在阐明知母（Anemarrhenae rhizoma）与天花粉（Trichosanthis radix）药对（AT）在2型糖尿病（T2DM）治疗中的药理特征与作用机制。基因本体（Gene Ontology，GO）与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）富集分析结果显示，AT可通过调控磷脂酰肌醇3-激酶/蛋白激酶B（PI3K/Akt）信号通路发挥抗2型糖尿病药理作用。随后分子对接实验证实，AT与核心靶点蛋白间具有较强结合亲和力。进一步体外（in vitro）实验证实，AT干预可显著降低体重、血糖与胰岛素水平，同时阻断PI3K/Akt信号通路的激活。

搜集汇总

背景与挑战

背景概述

该数据集基于网络药理学分析和动物实验，研究知母与天花粉（AT）药对治疗2型糖尿病的机制，发现AT通过调节PI3K/Akt信号通路降低血糖、体重和胰岛素水平。数据集发布于2025年，涵盖生物化学、医学和药理学等领域，使用开放获取许可（CC BY 4.0），为相关研究提供实验数据和药理分析支持。