Production and evaluation of gH proteins of different subtypes.

Evaluation of the gH vaccine technology for H1N1, H3N2, H5N1 influenza A and influenza B strains. gH proteins of the influenza strains which were the components of the conventional trivalent influenza vaccine of 2008/2009 namely A/Brisbane/59/07 (H1N1), A/Uruguay/716/07 (H3N2) and B/Brisbane/3/07 (influenza B) as well of two prototype H5N1 strains namely A/Vietnam/1203/04 and A/Indonesia/5/05 were produced and. Production yields of recombinant proteins after purification and refolding per liter fermentation are shown. Groups of BALB/c mice (n = 5) were subcutaneously immunized once with 10 µg of the gH proteins in Alum. IgG titers against native trimeric full-length HA of the individual strains were determined three weeks later.