Vriant call files; sequencing TP53 in corticotroph pituitary tumors using Ion AmpliSeq TP53 Panel

Variant call files were used for the anylysis of TP53 coding sequence in 128 corticotroph tumor samples as described in article Relevance of mutations inprotein deubiquitinases genes and TP53 in corticotroph pituitary tumors; DOI 10.3389/fendo.2024.1302667

The TP53 gene was sequenced by NGS using Ion Torrent technology. For this purpose, a pre-designed panel Ion AmpliSeq TP53 Panel (TP53.20140108, Ion AmpliSeq Community Panel) covering the entire protein-coding sequence of TP53 and exonintron boundaries was selected by the online tool Ion Ampliseq Designer (Thermo Fisher Scientific). 

Sample concentrations were measured using Qubit 2.0 Fluorometer (Life Technologies) with the dsDNA HS Assay Kit. Next, according to the manufacturer’s instructions, gene libraries were prepared using Ion AmpliSeq™ Library Kit Plus (Thermo Fisher Scientific) and the primers. Sequencing was performed using Ion GeneStudio™ S5 Prime System (Thermo Fisher Scientific).

Variant calling was performed using Ion Torrent Server software (Torrent Suite version 5.12.3)