Short-Exposure Transmission Electron Microscopy of Cilia

Noisy and pseudo ground-truth short-exposure transmission electron microscopy (TEM) images of Cilia used to obtain the results depicted in Fig. 3 in the paper "Zero-Shot Denoising of Microscopy Images Recorded at High-Resolution Limits" (Salwig & Drefs et al., 2024). The images were derived based on a dataset provided upon personal communication with the authors of the paper "Denoising of Short Exposure Transmission Electron Microscopy Images For Ultrastructural Enhancement" (Bajíc et al., 2018). The original dataset consisted of a sequence of 100 noisy short-exposure TEM images of a scene showing a cilium. The images had a resolution of 2048 × 2048 pixels, and each image depicted a slightly shifted version of the scene. The file pseudo-ground-truth.tif was obtained by first aligning all images of the sequence using rigid registration (Marstal et al., 2016) and subsequently computing the pixel-wise median (following a procedure discussed in Bajíc et al., 2018). The file noisy.tif was obtained by randomly selecting one image from the sequence (the 91st image). The images are stored in 16 bit TIF format. For visualization, use an image viewer capable of reading 16 bit images (e.g. ImageJ).