Slik Overexpression Induces Apoptosis

(A and B) Two views of one ptcGAL4 UAS-slik UAS-GFP wing disc. (A) Optical section in the columnar epithelial layer of the wing pouch. Red shows actin staining. Green shows ptcGAL4-expressing cells visualized by GFP. Blue shows DAPI staining. (B) Basal optical section to show extruded GFP+ cells with pyknotic nuclei. DAPI is shown below. (C–E) ptcGAL4 UAS-slik UAS-GFP UAS-p35 wing disc. The GFP-expressing stripe is wider. Basally extruded cells have morphologically normal nuclei (arrow). In (E), optical cross-section show a large accumulation of ptcGAL4 UAS-slik UAS-GFP UAS-p35 cells below the epithelial layer.