Figure.2.THP1
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To perform proof-of-concept experiment for SLA-detection of nuclear localization of NF-kB in TNFa-stimulated THP1 myeloid cell lines. Conclusion: NFkB nuclear import is successfully visualized using flow cytometry for THP-1 cell lines, with a 2-3 fold increase in signal upon stimulation. These results were substantiated by confocal microscopy experiments done in parallel, showing visually what SLA was able to quantify by flow cytometry. Notes: This experiment, along with additional experiments using the U-937 monocytic cell line demonstrates proof-of-concept for the SLA method. Negative control: no primary antibody Positive control: double-strand DNA - Histone H3 interaction Experiment: NFkB - dsDNA interaction
创建时间：
2017-01-01
