RT_Immunological Changes_interval. RT_Immunological Changes_interval

RT upregulated PD-L1 expression levels in tumor cells from the middle to late phase, which subsequently declined to a level comparable to baseline in the progression phase. When compared to 7.5 Gy, the frequency of PD-L1+ myeloid-derived suppressor cells with 2 Gy × 5 was maintained to be higher, showing tumor regression to a lesser extent. The proportion of PD-1+CD8+ and interferon-γ+CD8α T cells continued to increase until the end of the evaluation. The frequency of splenic PD-1+CD8+ T cells was significantly elevated following RT, and the effect was sustained longer with 2 Gy × 5. The transcriptomic data analysis revealed that RT stimulated the transcription of overall immunostimulatory and inhibitory genes, showing sequentially altered patterns. Overall design: Each time point has 0Gy, 2Gyx5, and 7.5Gy samples. PreRT sample is a non-irradiated control group.