File S1 - A Long QT Mutation Substitutes Cholesterol for Phosphatidylinositol-4,5-Bisphosphate in KCNQ1 Channel Regulation

Supporting Information Figures and Tables. Figure S1, Effect of cyclodextrin on WT and R539W activation kinetics. A, Representative recording of a COS-7 cell transfected with WT or R539W KCNE1-KCNQ1 concatemer channel, without and with 1-hour pre-treatment by 2 mmol/L cyclodextrin. B and C, Mean +/− sem of WT and R539W activation tau from monoexponential fit of activation without and with cyclodextrin pre-treatment. * pFigure S2, Effect of triparanol on WT and R539W activation kinetics. A, Representative recording of a COS-7 cell transfected with WT or R539W KCNE1-KCNQ1 concatemer channel, without and with 24-hour pre-treatment by 10 µmol/L triparanol. B and C, Mean +/− sem of WT and R539W activation tau from monoexponential fit of activation without and with triparanol pre-treatment. * pTable S1, Wortmannin-induced rundown. Table S2, Magnesium-induced rundown. Table S3, Ci-VSP-induced rundown. (PDF)