Effect of TGF-beta1 on the miRNome in CFBE41o- cells stably transduced with Wild Type (WT)-CFTR and F508del-CFTR

The purpose of this study was to explore miRNA mediated Transforming Growth Factor (TGF)-ß1 regulation of F508del Cystic Fibrosis Transmembrane Conductance regulator (CFTR). To fulfill this goal, miRNA sequencing was done to see miRNA landscape in Cystic Fibrosis Bronchial Epithelial (CFBE) Cells with homozygous WT-CFTR and F508del mutated CFTR in response to TGFß1 treatment. Overall design: Sequencing microRNAs in WT-CFBE41o- and F508delCFBE41o- cells after 24hour treatment with TGFß1.