Small scale RNAi screen testing juglone and arsenite tolerance.

Genes involved in the C. elegans GSH-metabolism were classified according to their reported or predicted functions. Each of the 67 listed genes was targeted by RNAi starting in L4 animals. F1 L4/young adults of the treated worms were transferred to 0.15 mM juglone and 3 mM arsenite stress plates, respectively, and the survival rates (± SD) were determined after 18 h at 20°C. Significance levels were determined by student’s t-test (n ≥3, at least 90 animals, *P<0.05, **P<0.01, and ***P<0.001). RNAi clones of the RNAi library (Geneservices, positions are shown) were verified by PCR using T7-primer followed by analytical restriction. Where indicated, RNAi constructs for genes that are not included in the library were generated by standard cloning of PCR products into the pL4440 vector, n.d. not determined.+genes possess different isoforms, however, all isoforms are affected by the RNAi constructs used.