Raw data of image quantification.

Each tab contains the raw data of quantifications presented in the manuscript. The first tab contains the quantifications depicted in Fig 1D and 1F, where the normalized fluorescence intensity of mCherry and GFP were calculated for diploids undergoing meiosis. Traces for each diploid were plotted as the mean with 95% confidence interval against time. The second tab contains the quantification depicted in Fig 2C and 2F of reporter (promoter-FP) expression in spores. For each ascus, we detail the mean fluorescence intensity of the 4 individual spores and the total of the four spores. We then divided each individual spore by the total to get the percent of total spore fluorescence inside a given spore. The third tab details the quantification depicted in Fig 4D of percent intensity inside spores of given fluorescent proteins. We show the percent of fluorescent protein inside spores in three different genotypes (ppoison-wtf4antidote-GFP/mCh-wtf4poison, ppoison-wtf4antidote-GFP/ade6+, mCh-wtf4poison/ade6+). The fourth tab details the Fluorescence Recovery After Photobleaching (FRAP) quantifications depicted in S3B Fig. We normalized the fluorescence intensity before bleaching to 1. Once bleaching was completed, the recovery was recorded every 10 minutes for approximately 6 hours. The fluorescence levels of both mCherry-Wtf4 and Wtf4poison-GFP are recorded for each time point. The fifth tab details the Fluorescence Recovery After Photobleaching (FRAP) quantifications depicted in S3D Fig. The sixth tab details the quantifications shown in S7C Fig, with the fluorescence intensity inside asci and diploids for the following constructs: pantidote-mCherry, pantidote long-mCherry, and the empty vector control. The seventh tab details Fig 5D, the percent intensity inside spores of given fluorescent proteins. We show the percent of Wtf4poison-GFP inside spores in three different genotypes: pantidote-wtf4poison-GFP/ade6+, wtf4antidote-mCh/ura4+; wtf4poison-GFP/ade6+; and wtf4poison-GFP/ade6+, wtf4antidote-mCh/ura4+. (XLSX)