Osteocytes regulate transcortical blood vessel network by transferring mitochondria to endothelial cells in cortical bone

In this study, mitochondria were proved to be capable of delivering mitochondria to endothelial cells of transcortical blood vessel and promotes its angiogenesis. By utilizing RNA seq, we investigated the effect of osteocytes-derived mitochondria on endothelial cells. Overall design: bEnd.3 cells were co-cultured with or without mitochondria isolated from MLO-Y4 cells in vitro. After 24 hours, bEnd.3 cells were collected for further RNA-Seq analysis.