Comparison of gut microbiome profiles related to anxiety-like behavior changes following hFMT

This study aimed to identify gut microbes that influence anxiety-like behavior by humanizing the gut microbiota of C57BL/6 mice through human fecal microbiota transplantation (FMT). Anxiety-like behaviors were assessed using the Light-Dark Box (LDB) before and after FMT to evaluate behavioral shifts. Based on post-FMT behavioral changes, mice were categorized into three groups: Low-Anxiety (LA), Intermediate (MA), and High-Anxiety (HA) groups. Gut microbiota composition was analyzed using samples collected from both feces and the whole gastrointestinal tract to compare microbial profiles and their correlation with anxiety-related phenotypes across groups.