The effect of beta-glucan - induced trained immunity on the transcriptomic profile of tumor-associated monocytes

Induction of trained immunity by beta-glucan has been shown to promote transcriptomic alterations in myeloid cells. Monocytes are involved in the immune responses against tumors. In this study, we performed RNA sequencing in tumor-infiltrated monocytes from mice in order to investigate the impact of trained immunity on the transcriptomic profile of monocytes in the tumor setting. Overall design: WT mice were pretreated with a single dose of 1 mg of ß-glucan from Trametes versicolor (Invivogen) in PBS or with PBS vehicle alone (control). After 7 days, mice were injected subcutaneously with 3 x 105 B16-F10 melanoma cells into the right flank. Monocytes (CD45+CD11c-CD11b+Ly6g-Ly6c+) that were infiltrated in the tumor were sorted 14 days after tumor cell injection and RNA sequencing analysis was performed.