Transcriptional profile of NOMO1 knockout cell lines [RNA-seq]

We report the data of high-troughput RNA sequencing of two different cell lines, HCT-116 (early-onset colorectal cancer; EOCRC) and HS-5 (bone marrow) in order to analyze the transcriptional profile generated after NOMO1 (Nodal Modulator 1) gene inactivation, a candidate biomarker in EOCRC. The generation of the NOMO1 knockout cell lines was performed using CRISPR/cas9 technology. The main objective was to identify whether the loss of NOMO1 generated a differential transcriptional profile in each cell line and whether it was possible to identify genes commonly deregulated in the two NOMO1-KO cell lines. RNA sequencing revealed a set of 592 differentially expressed genes (FDR<0.05) in common to the two cell lines as a consequence of NOMO1 inactivation. Overall design: Examination of the transcriptional profile of HCT-116 and HS-5 NOMO1 knockout cell lines.