RNA sequencing for S. rosetta cRFXa and foxJ1 mutated strains

The purpose of this data is to perform differential expression analysis comparing loss-of-function mutations in cRFXa and foxJ1 in S. rosetta, compared to reference wild-type strains. Separate wild-type sequencing data was collected for comparing to either cRFXa or foxJ1, since the foxJ1 mutated strain also contains a cycloheximide-resistance mutation in Rpl36a, so a strain containing only the cycloheximide resistance mutation was used as a reference wild-type strain for the foxJ1 mutant. For each strain, triplicate samples were collected from different cultures prepped on different days, each day collecting one wild-type and one mutant sample for lysis and RNA extraction. The purpose of this study is to assess whether either of these transcription factors regulates genes involved in ciliogenesis.