Effect of lysis intermixing on the predicted maturation order

Supplementary analyzed data from Manuscript:
TITLE: Maturation kinetics of a multiprotein complex revealed by metabolic labeling.
JOURNAL: CELL.
Article Type: Research Article
Authors: Evgeny Onischenko*, Elad Noor*, Jonas S. Fischer*, Ludovic Gillet, Matthias Wojtynek, Pascal Vallotton, Karsten Weis
*Equally Contributing Authors
Corresponding Authors: Evgeny Onischenko and Karsten Weis

Related to Figure S5

Possible maturation orders in NPC biogenesis, are represented by a binary trees whose leaves are the 9 identified NUP assembly groups. The leafs are allowed to gradually merge representing the co-assembly events among NUPs until they form a complete NPC (tree root). Each leaf and each vertex (i.e. a mature complex of several such leaves) in the tree is assigned a single variable which represents its relative pool size. For every possible tree configuration this set of variables is optimised to match the effective maturation pool data from the KSM fitting. RMSE (Root Mean Squared Error) between the computed maturation pools from the binary tree and the ones estimated by KSM is used as the tree score.

Maturation orders were assayed with the raw prey labeling values (Top trees (raw labeling values)) and with the values corrected for protein intermixing at the AP step (Top trees (intermixing-corrected labeling values)).