File S1 - Identification of a STAT5 Target Gene, Dpf3, Provides Novel Insights in Chronic Lymphocytic Leukemia

Supporting Information (Methods, Results, Discussion, References, Tables, Figures and Sequences). Methods S1: Library generation and analysis of sequences. Electrophoretic mobility shift DNA binding assays. Immunoblot Analysis. Results S1: Comparison of the methods used for optimization of ChIP and library generation. Comparison of the libraries generated for STAT5 target genes identification. Induction of expression of potential STAT5 target genes upon IL-3 stimulation. Confirmation of STAT5 binding to the selected target genes. Assessing the efficiency of STAT5 knock-downs. Discussion S1. References S1. Table S1: Primers-oligos used in the study. Table S2: Selected potential STAT5 target genes. Figure S1: Comparisons of efficiency of the methodologies used. Figure S2: Expression levels of selected STAT5a target genes. Figure S3: STAT5 binding to the novel target genes. Figure S4: Efficiency of STAT5a and STAT5b knock-downs. Figure S5: Immunofluorescence detection of activated STAT5 (p-STAT5) and DPF3 in CLL. Figure S6: Sequence of the human DPF3 promoter. Sequences S1: Sequences from the ChIP followed by streptavidin precipitation library. Sequences S2: Sequences from the double ChIP library. (DOCX)