The role of Phc1 in organizing the chromatin interactions of the Nanog locus in ESCs

To look into whether Phc1 is involved in organizing the chromatin interactions of the Nanog locus in ESCs. We performed circularized chromosome conformation capture sequencing (4C-seq) of the genome-wide regions interacting with the Nanog locus in both Phc1+/+ and Phc1-/- mESCs. By doing so, we showed that ablation of Phc1 impaired the genome-wide chromatin interactions of the Nanog locus in mESCs. 4C-seq of the genome-wide regions interacting with the Nanog locus in both Phc1+/+ and Phc1-/- mESCs.