Effects of SLS or urushiol on the whole-genome scale transcription of human epidermal keratinocytes.

To understand molecular pathogenesis of chemical induced contact dermatitis, cultured normal human epidermal keratinocytes (NHKCs) were treated with sub-cytotoxic concentration of sodium lauryl sulfate (SLS, 10 microM) and urushiol (1 microM). In analysis, 259 genes were selected as up-regulated DEGs in the SLS-treated NHKCs and 193 genes as down-regulated DEGs. In urushiol-treated NHKCs, 173 up-regulated and 124 down-regulated DEGs were identified. Gene Ontology (GO) biological process (BP) in the SLS-induced upregulated DEGS in NHKCs were inflammation-associated biological processes, such as inflammatory response (GO:0006954), cellular response to calcium ion (GO:0071277), immune response (GO:0006955) and cellular response to lipopolysaccharide (GO:0071222) and in urushiol-induced upregulated DEGs were inflammation-associated biological processes such as cytokine-mediated signaling pathway (GO:0019221) and inflammatory response (GO:0006954). SLS downregulated proteins in chemotaxis (GO:0006935), oxidation-reduction process (GO:0055114), cornification (GO:0070268), G1/S transition of mitotic cell cycle (GO:0000082), keratinization (GO:0031424) and cellular protein metabolic process (GO:0044267) and urushiol downregulated proteins in nervous system development (GO:0007399), positive regulation of cell proliferation (GO:0008284) and cellular protein metabolic process (GO:0044267) significantly. Normal Human Epidermal Keratinocytes (NHKCs) were treated with 10 microM of SLS or 1 microM of urushiol for 24 hours and harvested for total RNA extraction and hybridization on Affymetrix Human GeneChip U133 2.0 microarrays.