Fibroblasts-derived from Pluripotent Cells Harboring a Single Allele Knockout in Two Pluripotency Genes Exhibit DNA Methylation Abnormalities and Reprogramming Defects

Gene expression comparison between various pluripotent stem cell lines (as indicated below) that grew under 2i/Lif or S/Lif conditions Overall design: ESCs (V6.5), secondary iPSCs system with one allele of Nanog KO (NGFP2N+/-) or with additional KO in one allele in Sall4 (NGFP2N+/-; S+/-), Esrrb (NGFP2N+/-; E+/-) or Utf1 (NGFP2N+/-; U+/-) were grown under 2i/Lif or S/Lif conditions and subjected to RNA sequencing.