Transcriptional changes in mouse lungs induced by house dust mite
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https://www.ncbi.nlm.nih.gov/sra/SRP114764
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资源简介：
We examined the transcriptional changes in mouse lungs induced by house dust mite (HDM), D.f. extract and the isolated RNA was utilized for RNA-seq analysis. Our results demonstrated that HDM strongly triggered an inflammatory response. Overall design: Total RNA was isolated from the lung of wild type or TLR3MAVS double knockout mice that were treated with PBS or HDM (D.f.) extract for 14-16 hours. Illumina TruSeq RNA Sample Preparation was performed following manufacturer's protocol. Samples were run on an Illumina HiSeq 3000 . Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm9 whole genome using TopHat v2.0.8b. Genes were annotated and quantified by HTSeq-DESeq pipeline.
创建时间：
2019-10-31
