BMP gradient along the intestinal villus axis controls zonated enterocyte and goblet cell states

Single cell and bulk RNA sequencing was performed from human intestinal organoids differentiated for 5 days in the presence or absence of BMP-2 and BMP-4 ligands. Overall design: Single cell and bulk RNA sequencing was performed from human intestinal organoids differentiated for 5 days in the presence or absence of BMP-2 and BMP-4 ligands. For in vivo validation, intestines of wildtype and BMPR1a knockout mice were subjected to bulk RNA sequencing.