Assay for Transposase-Accessible Chromatin in E4BP4 overexpressed RAW264.7 cells

ATACseq was performed by overexpressing E4BP4 in RAW264.7 cells using lentivirus. Lentivirus expressing mCherry(control) or E4BP4 were produced by Vector Builder.RAW264.7 cells were plated at a density of 1x105 cells/well (non-treated 24-well plate). 24 hours　after incubation, RAW cells were infected with lentiviruses expressing mCherry or E4BP4 at 100 MOI. After infection, the cells were replaced with a fresh medium, and 48 hours later, the cells were collected and used for ATAC-seq.