MS2-affinity purification coupled with RNA sequencing (MAPS) reveals CyaR sRNA targetome in Escherichia coli.

Recently, we developed an in vivo technology to draw the interacting map of a specific small regulatory RNA (sRNA). We called it MAPS for MS2-affinity purification coupled with RNA sequencing. Using this technology, we already revealed the targetome of RyhB, RybB and DsrA, three well-characterized sRNAs in Escherichia coli. In this study, we performed MAPS with CyaR sRNA. Overall design: Identification of RNAs co-purified with MS2-CyaR in a rne131 ?cyaR strain. CyaR (without MS2) was used as control.