Chan-Soo Lee, Chang-Ki Choi, Eun-Young Shin, Martin Alexander Schwartz, Eung-Gook Kim (2011) CIL:26548, Mus musculus, fibroblast. CIL. Dataset

To study the molecular mechanism by which nonmuscle myosin II (MII) regulates protrusion and adhesion dynamics in migrating cells, NIH3T3 cells were treated with βPIX siRNA for 2 days, treated with blebbistatin (BBS) for 30 min., and stained for βPIX (green) and actin (red). These findings help elucidate a functional link between MII and Rac1/Cdc42 GTPases, which may regulate protrusion/adhesion dynamics in migrating cells. This image is the original data file from Fig. 6B, “Requirement for βPIX in MII-regulated cell protrusion and adhesion.” J. Cell Biol. 2010. Vol. 190(4):663–674.

为探究非肌肉肌球蛋白II（MII）调节迁移细胞突起和粘附动力学之分子机制，NIH3T3细胞经过βPIX siRNA处理2日，随后用裂肌菌素（BBS）处理30分钟，并分别对βPIX（绿色）和肌动蛋白（红色）进行染色。本研究揭示了MII与Rac1/Cdc42 GTP酶之间的功能联系，这些酶可能调节迁移细胞中的突起/粘附动力学。本图即为图6B“βPIX在MII调节细胞突起和粘附中的必要性”的原数据文件，来源：J. Cell Biol. 2010，Vol. 190(4)：663–674。