Comparative Analysis of Liver Transcriptomes Associated with Hypoxia Tolerance and Bortezomib in Megalobrama amblycephala

Megalobrama amblycephala is important economic fish, but it is sensitive to hypoxia. A new Megalobrama amblycephala strain, pujiang No 2, was developed by Shanghai Ocean University to overcome this shortcoming. In the present study, bortezomib was used to explore the hypoxia adaptation mechanism of pujiang No 2. We conducted the microstructure observation and the comparative analyses of the liver transcriptomes of HN, HB, NN. KEGG pathway analysis showed that many DEGs were enriched in the FOXO signaling pathway, NF kappa B signaling pathway, and MAPK signaling pathway. In addition, we found that treatment with bortezomib might aggravate the hypoxic stress response of pujiang No 2. The results of this study suggested genes and signaling pathways associated with the molecular mechanisms of hypoxia tolerance in Pujiang No 2 and were of great significance for fish genetic breeding.