Data from: Exhaustive extraction of cyclopeptides from Amanita phalloides: guidelines for working with complex mixtures of secondary metabolites

Understanding plant-insect interactions is an active area of research in
both ecology and evolution. Much attention has been focused the impact of
secondary metabolites in the host plant or fungi on these interactions.
Plants and fungi contain a variety of biologically active compounds, and
the secondary metabolite profile can vary significantly between individual
samples. However, many experiments characterize the biological effects of
only a single secondary metabolite or a subset of these compounds. Here,
we develop an exhaustive extraction protocol using an accelerated solvent
extraction protocol to recover the complete suite of secondary metabolites
found in Amanita phalloides (death cap mushrooms) and compare its efficacy
to the ‘Classic’ extraction method used in earlier works. We demonstrate
that our extraction protocol recovers the full suite of secondary
metabolites in A. phalloides unlike the ‘Classic’ method that favors polar
secondary metabolites. Based on these findings, we provide recommendations
for how to optimize protocols to ensure exhaustive extracts and also the
best practices when using natural extracts in ecological experiments.