RNASeq of bone marrow-derived macrophages treated with beta-glucan and lipopolysaccharide (LPS)

We found that beta-glucan treatment induces early induction of transcripts associated with inflammation and metabolism. Several days after beta-glucan treatment, genes associated with differentation and housekeeping remain upregulated. LPS treatment induces a strong inflammatory response. Beta-glucan did not significantly alter the macrophage response to LPS. Overall design: Bone marrow derived macrophages were harvested for RNA after 4hrs of beta-glucan treatment, 24hrs of beta-glucan treatment, and 3 days following the end of treatment (3dp). Additionally, macrophage RNA was harvested after 3dp beta-glucan treatment and restimulation with LPS for 6hrs.