RNAseq analysis of genes activated 90 minutes, or 7 days post irradiation or untreated cells with knockdown of NFKBIA

Goal of the study was to investigate genes regulated differentially after 90 minutes versus 7 days after irradiation. 7 day time point corresponds to cellular senescence. U2OS cells were irradiated with 20 Gy and harvested either 90 minutes or 7 days. In addition cells were left untreated (control) or had knockdown of NFKBIA (protein name Ikappa B alpha). Ikappa B alpha is the main inhibitor of the transcription factor NFkappa B. NFkappa B regulates transcription of many factors constituting the SASP (senescence associated secretory phenotype).